油壓式胚胎顯微注射器
NANOLITER2010 Nanoliter 2010 Injector
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Injection volumes
WPI's microprocessor-controlled Nanoliter 2010 uses direct piston displacement. By either pushing the injection button on the control box or pressing on the optional footswitch, a calibrated volume will be smoothly injected. The process is quiet and vibration free. Capillary filling and injection speeds are 23 nL/sec and 46 nL/sec (emptying speeds are 92 nL/sec and 230 nL/sec). Maximum fluid ejection is 5 µL. Each unit comes with sufficient glass to pull at least 300 tips. Glass is 1.14 mm O.D. (nominal) and 0.5mm I.D. By setting the DIP switch, the injection volume can be changed from 2.3 to 69.0 nL in 16 steps. Up to 100 injections may be triggered per filling. Since the volume of a normal Xenopus oocyte is about 500 nL, the instrument has the capability to inject from less than 1% to over 10% of the total volume of the oocyte in one preset step increment.
Included with pump
Included: 1 vial 3.5 in. capillaries (300); replacement "O" rings; Allen wrench; MicroFil™ MF34G backfilling needle; and two sample pre-pulled micropipettes.
Smart controller
MICRO4, an optional microprocessor-based controller, can provide an "intelligent" and easy-to-use interface to up to four Nanoliter Injectors. Operating parameters are set with the membrane key-pad and LCD display. From the keypad the user can set pump to infusion or withdrawal mode, enter the volume to be infused or withdrawn, and rate of delivery, as well as synchronize the starting and stopping of a combination of Injectors. User parameters can be stored in the device's "non-volatile" memory for instant recall when the unit is powered on. An optional footswitch can be plugged into a connector on the rear of the controller for "hands free" start/stop operation. An RS-232 port on the rear of the controller can be used to connect it to a computer for use with computer control programs.
WPI's microprocessor-controlled Nanoliter 2010 uses direct piston displacement. By either pushing the injection button on the control box or pressing on the optional footswitch, a calibrated volume will be smoothly injected. The process is quiet and vibration free. Capillary filling and injection speeds are 23 nL/sec and 46 nL/sec (emptying speeds are 92 nL/sec and 230 nL/sec). Maximum fluid ejection is 5 µL. Each unit comes with sufficient glass to pull at least 300 tips. Glass is 1.14 mm O.D. (nominal) and 0.5mm I.D. By setting the DIP switch, the injection volume can be changed from 2.3 to 69.0 nL in 16 steps. Up to 100 injections may be triggered per filling. Since the volume of a normal Xenopus oocyte is about 500 nL, the instrument has the capability to inject from less than 1% to over 10% of the total volume of the oocyte in one preset step increment.
Included with pump
Included: 1 vial 3.5 in. capillaries (300); replacement "O" rings; Allen wrench; MicroFil™ MF34G backfilling needle; and two sample pre-pulled micropipettes.
Smart controller
MICRO4, an optional microprocessor-based controller, can provide an "intelligent" and easy-to-use interface to up to four Nanoliter Injectors. Operating parameters are set with the membrane key-pad and LCD display. From the keypad the user can set pump to infusion or withdrawal mode, enter the volume to be infused or withdrawn, and rate of delivery, as well as synchronize the starting and stopping of a combination of Injectors. User parameters can be stored in the device's "non-volatile" memory for instant recall when the unit is powered on. An optional footswitch can be plugged into a connector on the rear of the controller for "hands free" start/stop operation. An RS-232 port on the rear of the controller can be used to connect it to a computer for use with computer control programs.
Nanoliter2010_ims.pdf | |
File Size: | 405 kb |
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WPI 氣動式胚胎顯微注射器 Pneumatic PicoPump
SYS-PV820 Pneumatic PicoPump
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Intracellular injection
Repeatable microinjection in volumes ranging from picoliters to nanoliters. Separate ports supply positive and negative pressure - positive pressure for high-pressure ejection, and suction for supporting the cell or for filling the pipette from the tip. A second pressure port maintains a low positive "holding" pressure to the injecting pipette between injection pulses, to prevent fluid uptake through capillary action or diffusion. Timing, ejection pressure, holding pressure, and suction are adjusted independently by control knobs and indicator gauges on the front panel. Injection pressure is controlled by a 20-turn regulator on the front panel. A built-in timing circuit allows precise control of the amount of time that the injection pressure is applied to the output port. Time intervals can range from 10 seconds down to 10 ms or less, depending on the eject pressure setting. The injection pressure interval can be triggered manually on the front panel, by footswitch, or by computer controlled TTL pulse. A 5 volt monitor output provides a logic-level pulse for your computer or other monitoring device.
PV820 Features
Separate regulated Hold and Ejection pressure, used to maintain a low pressure in the pipette between injections to prevent unwanted fluid uptake by capillary action or diffusion. A precision timing circuit switches from Eject pressure to Hold pressure automatically, once timing has been set. Although regulated vacuum is not provided in this model, suction can be provided by connecting a vacuum source to the vacuum port on the rear panel. Suction is then available through the pressure ports.
Repeatable microinjection in volumes ranging from picoliters to nanoliters. Separate ports supply positive and negative pressure - positive pressure for high-pressure ejection, and suction for supporting the cell or for filling the pipette from the tip. A second pressure port maintains a low positive "holding" pressure to the injecting pipette between injection pulses, to prevent fluid uptake through capillary action or diffusion. Timing, ejection pressure, holding pressure, and suction are adjusted independently by control knobs and indicator gauges on the front panel. Injection pressure is controlled by a 20-turn regulator on the front panel. A built-in timing circuit allows precise control of the amount of time that the injection pressure is applied to the output port. Time intervals can range from 10 seconds down to 10 ms or less, depending on the eject pressure setting. The injection pressure interval can be triggered manually on the front panel, by footswitch, or by computer controlled TTL pulse. A 5 volt monitor output provides a logic-level pulse for your computer or other monitoring device.
PV820 Features
Separate regulated Hold and Ejection pressure, used to maintain a low pressure in the pipette between injections to prevent unwanted fluid uptake by capillary action or diffusion. A precision timing circuit switches from Eject pressure to Hold pressure automatically, once timing has been set. Although regulated vacuum is not provided in this model, suction can be provided by connecting a vacuum source to the vacuum port on the rear panel. Suction is then available through the pressure ports.
PV820_Manual.pdf | |
File Size: | 355 kb |
File Type: |
氣動式胚胎顯微注射器 Pneumatic PicoPump with vacuum
SYS-PV830 Pneumatic PicoPump with vacuum
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Intracellular injection
Designed to simplify intracellular injection and a variety of other microinjection tasks, WPI's PicoPumps use carefully regulated air pressures for securing cells and injecting them with fluid. Injected volumes range from picoliters to nanoliters. Separate ports supply positive and negative pressure - positive pressure for high-pressure ejection, and suction for supporting the cell or for filling the pipette from the tip. A second pressure port maintains a low positive "holding" pressure to the injecting pipette between injection pulses, to prevent fluid uptake through capillary action or diffusion. Timing, ejection pressure, holding pressure, and suction are adjusted independently by control knobs and indicator gauges on the front panel. Injection pressure is controlled by a 20-turn regulator on the front panel. A built-in timing circuit allows precise control of the amount of time that the injection pressure is applied to the output port. Time intervals can range from 10 seconds down to 10 ms or less, depending on the eject pressure setting. The injection pressure interval can be triggered manually on the front panel, by footswitch, or by computer controlled TTL pulse. A 5 volt monitor output provides a logic-level pulse for your computer or other monitoring device.
PV830 Features
Eject pressure, Hold pressure, and Vacuum are all available, controlled by separate regulators on the front panel. Eject pressure supplies a high-pressure pulse for injecting fluid. Hold pressure, which is not sufficient to cause fluid ejection, is used to prevent back filling of the pipette by capillary action or diffusion when the solenoid is inactive. Pressure in the injection pipette is automatically switched between Eject and Hold pressure by a precision timing circuit that controls a solenoid valve. Vacuum is used to fill pipettes from the tip or to secure a floating cell during microinjection. Vacuum is regulated the same way, by a 20-turn knob on the front panel. Vacuum may be switched from regulated vacuum to atmosphere by using the pneumatic toggle on the front panel. Vacuum can also be routed to the eject port.
Designed to simplify intracellular injection and a variety of other microinjection tasks, WPI's PicoPumps use carefully regulated air pressures for securing cells and injecting them with fluid. Injected volumes range from picoliters to nanoliters. Separate ports supply positive and negative pressure - positive pressure for high-pressure ejection, and suction for supporting the cell or for filling the pipette from the tip. A second pressure port maintains a low positive "holding" pressure to the injecting pipette between injection pulses, to prevent fluid uptake through capillary action or diffusion. Timing, ejection pressure, holding pressure, and suction are adjusted independently by control knobs and indicator gauges on the front panel. Injection pressure is controlled by a 20-turn regulator on the front panel. A built-in timing circuit allows precise control of the amount of time that the injection pressure is applied to the output port. Time intervals can range from 10 seconds down to 10 ms or less, depending on the eject pressure setting. The injection pressure interval can be triggered manually on the front panel, by footswitch, or by computer controlled TTL pulse. A 5 volt monitor output provides a logic-level pulse for your computer or other monitoring device.
PV830 Features
Eject pressure, Hold pressure, and Vacuum are all available, controlled by separate regulators on the front panel. Eject pressure supplies a high-pressure pulse for injecting fluid. Hold pressure, which is not sufficient to cause fluid ejection, is used to prevent back filling of the pipette by capillary action or diffusion when the solenoid is inactive. Pressure in the injection pipette is automatically switched between Eject and Hold pressure by a precision timing circuit that controls a solenoid valve. Vacuum is used to fill pipettes from the tip or to secure a floating cell during microinjection. Vacuum is regulated the same way, by a 20-turn knob on the front panel. Vacuum may be switched from regulated vacuum to atmosphere by using the pneumatic toggle on the front panel. Vacuum can also be routed to the eject port.
PV830_Manual.pdf | |
File Size: | 1373 kb |
File Type: |